Enzymes
We offer a broad selection of enzymes suitable for research and development, production and diag- nostics. Our enzyme catalog contains innovative biocatalysts at desired quality grades to solve customer specific problems at best performance to their satisfaction.

Enzymicals AG has a unique feature in the recombinant pig liver esterases, (R)-selective amine transaminases and Baeyer-Villiger mono- oxygenases.

Further information on our enzymes

Carboxylesterases
Esterases represent a diverse group of hydrolases catalysing the cleavage and formation of ester bonds. Carboxylesterases are a subset of esterases that specifically hydrolyse carboxylic esters yielding a carboxylic acid and an alcohol.
Many of these enzymes show a high regio- and stereoselectivity, accept a broad spectrum of substrates and are stable and even active in the presence of organic cosolvents. Together with the fact that they do not require any cofactors this makes them attractive biocatalysts for the production of optically pure compounds for the synthesis of fine chemicals.
Within our catalog you can find esterases which are thermostable and/or accept carboxylic esters of tertiary alcohols, which is a rather rare feature.
We also offer six isoenzymes of the pig liver esterase which has been used in organic chemistry for decades already. The advantage of the recombinant production of single isoenzymes over the so far used extract from animal tissue lies in the purity and the reproducibility from batch to batch.

example reactions for regio- and stereoselectivity of esterases

Baeyer-Villiger monooxygenases
Baeyer-Villiger monooxygenases (BVMOs) are flavoenzymes and belong to the the class of oxidoreductases. They catalyse the oxidation of linear, cyclic and aromatic ketones to the corresponding esters or lactones, highly similar to the chemical Baeyer-Villiger oxidation. During the enzymatic oxidation one atom of molecular oxygen is incorporated into a carbon-carbon bond of a non-activated ketone.
Besides that they are also capable of other oxidations. For example they can oxidise several heteroatoms. Depending on the structure of the substrate the might even prefer the "side reaction" over the Baeyer-Villiger oxidation.
The BVMOs in our catalog require NADPH as cofactor and accept a variety of different substrate groups. We also offer thermostable BVMOs.

example reactions for BVMO conversions

examples for BVMO-catalysed "side reactions"
R are unspecified different residues

Amine transaminases
Amine transaminases (ATAs) catalyse the transfer of an amino group from a primary amine to a prochiral ketone (or aldehyde). Amine transaminases are highly enantioselective and therefore can be used for the resolution of racemic amines or the synthesis of chiral amines from inexpensive ketones.
ATAs are pyridoxal-5'-phosphate dependent enzymes and their substrate range includes aliphatic and aromatic amines, amino acids, amino esters and amino alcohols. Typical amine donors are alanine, ethylamine, 1- and 2-propyl- or butylamine and others.

example reaction for an ATA conversion

Aminoacylases
Aminoacylases hydrolyse the amide bond in N-acylated L-amino acids and are used for the preparation of enantiopure amino acids.
They require no cofactor.

aminoacylase-mediated stereoselective cleavage of a benzoylated amino acid